Here is your Tissue Culture Lab Startup Checklist in a printable and organized format, categorized by zone with space for checking and notes. It can be used for procurement planning, inventory, or facility setup.
For: Agarwood / Plant Issue Culture Lab (2-Unit Layout) 🧪 Total Space: 64 sqm (2 units of 4m x 8m) 📋 Prepared for: Putz Agarwood Farm or affiliate labs 📦 Use: Procurement planning, budget projection, and setup execution
I. MEDIA PREPARATION & STERILIZATION AREA
Item Description
Qty
Check
Notes
Weighing balance (0.001g precision)
1
☐
pH meter / pH strips
1
☐
Hot plate with magnetic stirrer
1
☐
Distilled/deionized water unit
1
☐
Autoclave (25–75 L capacity)
1
☐
Microwave oven
1
☐
Refrigerator (4°C)
1
☐
Beakers (100–1000 mL)
Set
☐
Measuring cylinders (50–500 mL)
Set
☐
Erlenmeyer flasks (250 mL–2L)
Set
☐
Test tubes & racks
Set
☐
Media bottles (500 mL–1 L, autoclavable)
Set
☐
Funnels, pipettes, droppers
Set
☐
Parafilm® / vessel caps
Roll
☐
Drying racks, plastic tubs, basins
Set
☐
Chemical & glassware storage cabinet
1
☐
II. INOCULATION ROOM (CLEAN AREA)
Item Description
Qty
Check
Notes
Laminar flow cabinet (HEPA)
1–2
☐
UV germicidal lamp (for LFC)
1
☐
Alcohol / spirit lamp
1–2
☐
Air purifier (HEPA)
1
☐
Air conditioner / climate control
1
☐
Digital thermometer/hygrometer
1
☐
Forceps (straight, curved)
Set
☐
Scalpels / blades
Set
☐
Dissecting scissors
1
☐
Inoculation table
1
☐
Gloves (sterile), masks, gowns
Box
☐
Sterile tissue wipes
Box
☐
Alcohol sprayers (70% EtOH)
2+
☐
🌱 III. CULTURE GROWTH ROOM
Item Description
Qty
Check
Notes
Multi-level culture racks
2–4
☐
LED grow lights w/ timers
1/set
☐
Air conditioner or climate control
1
☐
Hygrometer / thermometer
1
☐
Optional: Incubator (25–30°C range)
1
☐
Transparent vessels (jars, bottles)
200+
☐
Polycarbonate containers (w/ filters)
100+
☐
🧼 IV. CONSUMABLES & RAW MATERIALS
Item Description
Qty
Check
Notes
Agar powder / gellan gum
1kg
☐
Sucrose / glucose
5kg
☐
Murashige and Skoog (MS) salts
1L / set
☐
Plant growth regulators (PGRs)
Set
☐
Auxins, Cytokinins, GA
Vitamins (thiamine, myo-inositol, etc.)
Set
☐
NaOCl (bleach) for sterilization
4L
☐
Isopropyl or ethyl alcohol (70%)
4L
☐
Gloves, masks, gowns
Box
☐
Labels, pens, logbook
Set
☐
🔧 V. OPTIONAL ADVANCED EQUIPMENT
Item Description
Qty
Check
Notes
PCR / biosafety cabinet
1
☐
For DNA work
Stereo microscope
1
☐
For shoot-tip or embryo
Spectrophotometer
1
☐
For media analysis
Fume hood
1
☐
For volatile chemicals
Lab management software
1
☐
For tracking & inventory
Would you like this checklist saved as:
✅ Printable PDF
✅ Excel (.xlsx) for editable procurement tracking
✅ PowerPoint format for presentation use Let me know your preference and I’ll generate it for you.
