Microbial Enzyme-Based

Induction Techniques | BioFusion™ | FusaBlaze™

This method uses specific microbes and their enzymes to induce stress in Aquilaria trees, triggering natural defense responses that result in resin production.

Unlike traditional artificial methods (drilling + chemicals), this technique leverages bio-induction through microbial activity.

How It Works:

Microorganism Selection:

  • Beneficial microbes (especially fungi or bacteria like Fusarium oxysporum, Phaeoacremonium spp., or Trichoderma) are isolated and cultured.
  • These strains are selected for their ability to produce hydrolytic enzymes (like cellulase, ligninase, pectinase).

Enzyme Production:

  • The microbes produce enzymes that degrade or alter plant cell walls in targeted areas.
  • This enzymatic activity mimics natural infection or decay, triggering a defensive resin response in the tree.

Application/Inoculation:

  • The microbial-enzyme mix is applied via:
    • Small drill holes or bark incisions
    • Pressure-injection systems
    • Surface sprays (in some experimental cases)
  • Often done during the rainy season for optimal microbial activity.

Resin Accumulation:

  • Over 3–12 months, the infected area shows increased resin formation, darkening of wood, and aroma development.
  • The resulting agarwood has similar chemical profiles to wild-formed resin, rich in sesquiterpenes and chromones.

Advantages:

FeatureBenefit
Targeted InductionResin forms where enzymes act — efficient and controllable.
Bio-BasedEnvironmentally friendly, no toxic chemicals used.
High-Quality OutputResin similar to naturally infected trees — higher market value.
SustainableReduces over-harvesting and mimics natural processes.

Common Enzymes Involved:

EnzymeRole
CellulaseBreaks down cellulose, weakens cell walls.
LaccaseOxidizes phenols, facilitates resin polymerization.
PectinaseDegrades pectin in middle lamella, aiding penetration.
XylanaseAssists in hemicellulose breakdown.

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