Chemical Induction | SaltShock™ Inoculant | SaltShock™ Formulation | SaltShock™ Development | SaltShock™ Profile
Standard Operating Procedure (SOP) for the Sodium Chloride (NaCl) inoculant, used as an abiotic stressor to induce agarwood resin formation in Aquilaria spp.
SOP: NaCl-Based Agarwood Inoculant
Product Name: BarIno™ SaltStrike™
Tagline: “Stress it smart. Scent it strong.”
Purpose: To prepare a low-cost, moderately effective abiotic stress inoculant for inducing resin in agarwood trees via NaCl osmotic and ionic stress.
1. Materials Needed
| Material | Specification |
|---|---|
| Sodium Chloride (NaCl) | Non-iodized, food or lab grade (≥98%) |
| Distilled or clean water | Neutral pH (6.5–7.5) |
| Brown sugar (optional) | Organic or refined, powder form |
| Clean mixing container | Plastic or glass (non-metallic) |
| Stirring rod or hand mixer | Non-reactive (e.g., glass or plastic) |
| Measuring tools | Digital scale, graduated cylinder |
| PPE | Gloves, goggles, mask |
2. Formulation Procedure
Standard Formula:
- NaCl concentration: 3–5% w/v
(i.e., 30–50 g NaCl per liter of water) - Optional brown sugar: 2% w/v
(i.e., 20 g per liter) for microbial synergy
Preparation Steps:
- Weigh Ingredients:
- NaCl: 30 g (mild) to 50 g (moderate stress) per 1 L
- Brown sugar: 20 g (optional)
- Dissolve Solutes:
- Add ~900 mL distilled water into the container.
- Slowly add NaCl while stirring to dissolve fully.
- If using brown sugar, add and stir until clear.
- Adjust Final Volume:
- Add more water to make exactly 1 liter of solution.
- Filter (optional):
- For precision or sterile conditions, pass solution through cheesecloth or fine filter.
3. Storage & Shelf Life
| Parameter | Details |
|---|---|
| Storage Container | Amber glass or HDPE plastic bottle |
| Room Temp Shelf Life | 3–5 days (without sugar) |
| Refrigerated Shelf Life | 24–48 hrs (with sugar, to avoid microbial growth) |
| Label Requirements | Product name, batch no., concentration, date |
4. Application Guidelines
| Parameter | Specification |
|---|---|
| Tree age | ≥ 4 years (Aquilaria malaccensis, etc.) |
| Injection site | 6–8 mm boreholes, 5–7 cm deep |
| Volume per hole | 3–5 mL |
| Hole spacing | 10–15 cm vertical distance |
| Application schedule | One-time or repeat after 6–9 months |
5. Safety & Handling
- Wear gloves, goggles, and mask when mixing and applying.
- Avoid direct contact with eyes or prolonged skin exposure.
- Ensure containers and tools are clean to prevent contamination.
- Dispose of unused solution in salt-tolerant disposal areas or per local environmental rules.
6. Sample Field Record Template
| Date | Tree ID | Volume Used | Dose % | Notes (Color/Resin/Aroma) |
|---|---|---|---|---|
| YYYY-MM-DD | T001 | 5 mL/hole | 5% | Light brown zone, sweet smell |