Chemical Induction | ResinRush™ | ResinRush™ Formulation | ResinRush™ Development | ResinRush™ Profile
SOP (Standard Operating Procedure) for preparing and applying BarIno™ ResinRush™, a chemical agarwood inoculant that uses Ferric Chloride (FeCl₃) and Sodium Chloride (NaCl) to stimulate resin formation in Aquilaria trees.
This formulation works by inducing oxidative stress (via FeCl₃) and osmotic stress (via NaCl) to activate defense-related metabolic pathways, triggering artificial agarwood resin production.
FeCl₃ + NaCl Resin Induction Formula
I. OBJECTIVE
To prepare and apply a dual-stress agarwood inoculant to stimulate high-quality resin production through chemical means.
II. FORMULATION (Per 1 Liter Solution)
| Ingredient | Function | Amount |
|---|---|---|
| Ferric Chloride (FeCl₃), anhydrous or hexahydrate | Oxidative stress inducer | 20–25 g |
| Sodium Chloride (NaCl, common salt) | Osmotic stress inducer | 30–40 g |
| Di-ionized Water | Solvent/Base | to make 1,000 mL |
Optional: Add 0.1% Tween 20 or Tween 80 for better tissue penetration.
III. MATERIALS NEEDED
- Digital scale (±0.1 g precision)
- Clean 1-L glass or HDPE container
- Glass stirrer or non-metallic spoon
- Distilled or deionized water
- Measuring cylinder
- Personal Protective Equipment (gloves, goggles, lab coat)
- Labeling tape and marker
IV. PREPARATION PROCEDURE
- Prepare Base Water:
- Measure ~800 mL di-ionized water into a non-metallic container.
- Dissolve FeCl₃:
- Weigh 20–25 g of Ferric Chloride, slowly add to water while stirring.
- Stir until fully dissolved. (FeCl₃ is corrosive and exothermic when dissolved.)
- Add Sodium Chloride:
- Weigh 30–40 g NaCl, add to solution and stir until fully dissolved.
- Add Surfactant (Optional):
- Add 1 mL of Tween 20 or Tween 80 to enhance tissue penetration.
- Adjust Volume:
- Add di-ionized water to make up exactly 1,000 mL.
- Label and Store:
- Label container with product name, batch, and preparation date.
- Store in a cool, dark place. Use within 3–6 months for optimal effectiveness.
V. APPLICATION PROTOCOL
| Parameter | Details |
|---|---|
| Tree Age | 5 years old and above (≥10 cm DBH) |
| Application Site | Trunk and/or major branches |
| Drilling Pattern | Spiral or horizontal band; 15–20 cm apart |
| Hole Size | 6–8 mm diameter, 4–6 cm depth |
| Dose per Hole | 10–20 mL |
| Tool | Syringe or pressure injector |
| Reinoculation | Optional after 6–12 months |
| Post-Inoculation | Plug holes with sterile cotton or wax (optional) |
VI. SAFETY & HANDLING
- Wear gloves, eye protection, and long sleeves during handling.
- Avoid inhalation and direct skin/eye contact.
- Use in a well-ventilated area.
- Rinse all tools and containers with di-ionized water after use.
- Dispose of waste solution per local environmental regulations.
VII. DOCUMENTATION / LOGBOOK
Record the following for each batch and application:
- Date of preparation
- Batch number
- Tree ID / Location
- Volume applied per tree
- Observations after 3, 6, 12 months