DNA Barcode

Aquilaria malaccensis that we have in our nursery matched 100% with reference database when the sequences ran. Simply means, what we propagated, distributed, cultivated and planted in our farm is the correct and certified species of Aquilaria malaccensis (BARI).

Specimens sample used has been kept in our arboretum.

DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that, by comparison with a reference library of such DNA sections (also called “sequences”), an individual sequence can be used to uniquely identify an organism to species, in the same way that a supermarket scanner uses the familiar black stripes of the UPC barcode to identify an item in its stock against its reference database. These “barcodes” are sometimes used in an effort to identify unknown species, parts of an organism, or simply to catalog as many taxa as possible, or to compare with traditional taxonomy in an effort to determine species boundaries.


Species identification and taxonomic assignment

The taxonomic assignment of the OTUs to species is achieved by matching of sequences to reference libraries. The Basic Local Alignment Search Tool (BLAST) is commonly used to identify regions of similarity between sequences by comparing sequence reads from the sample to sequences in reference databases. If the reference database contains sequences of the relevant species, then the sample sequences can be identified to species level. If a sequence cannot be matched to an existing reference library entry, DNA barcoding can be used to create a new entry.

In some cases, due to the incompleteness of reference databases, identification can only be achieved at higher taxonomic levels, such as assignment to a family or class. In some organism groups such as bacteria, taxonomic assignment to species level is often not possible. In such cases, a sample may be assigned to a particular operational taxonomic unit (OTU).