Xylanase Enzyme

Microbial Enzyme | Cellulase | Laccase | Xylanase | Pectinase | Peroxidase

Xylanase – Hemicellulose-Degrading Enzyme for Agarwood Inoculation

Xylanase is a group of enzymes that break down xylan, a major component of hemicellulose in plant cell walls, into xylose and oligosaccharides. It plays an important supportive role in agarwood inoculation by enhancing tissue permeability and facilitating deeper fungal colonization and resin spread.

Scientific Overview

• Enzyme Class: EC 3.2.1.8
• Action: Hydrolyzes β-1,4-xylosidic linkages in xylan
• Source:
  • Fungi: Trichoderma reesei, Aspergillus niger
  • Bacteria: Bacillus subtilis, Streptomyces spp.
  • Commercial-grade: Food and fermentation industry

Role in Agarwood (Aquilaria) Inoculation

Functions:

Role	Benefit
Breaks down hemicellulose	Opens up xylem and fibers for inoculant access
Tissue softening	Enhances uptake and spread of fungal/abiotic agents
Stimulates defense response	Causes cellular disruption → host stress signaling
Synergizes with cellulase/laccase	Allows deeper colonization and improved resin formation

Synergy With Other Agents:

• Xylanase + Cellulase: Breaks down total plant wall polysaccharides.
• Xylanase + Laccase: Xylanase opens tissues, laccase oxidizes phenolics.
• Xylanase + Fusarium oxysporum: Helps the fungus invade host xylem and pith layers.

Application Protocol (Field Use)

Xylanase Pre-Treatment (Per Tree):

Item	Dosage
Xylanase	10–20 mg (≥ 1000 U/g activity)
Sterile water	10–20 mL
Injection	2–3 mL/hole into 3–5 holes

• Apply 6–12 hours before fungal inoculation (e.g., Fusarium oxysporum)
• Follow with BarIno™ or fungal inoculant
• Seal holes after co-injection

Optimal Conditions

Parameter	Value
pH	5.0–6.0
Temperature	30–50°C
Stability	Moderate; avoid UV and high alkalinity

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