Induction | Microbial Enzyme | Formulation Matrix | BioFusion™ | FusaBlaze™ | MycoChem™
Formulation Matrix of Enzyme Blends for Agarwood malaccensis Inoculation, tailored by tree age and physiological status. This guide helps select the best combination of cell wall–degrading and resin-enhancing enzymes to support fungal colonization and oleoresin formation across different growth stages.
Enzyme Blend Formulation Matrix by Tree Age
| Tree Age | Tree Status | Recommended Enzyme Blend | Purpose | Notes |
|---|---|---|---|---|
| 2–3 years | Young, immature xylem | –Mild cellulase (5 mg) – Low xylanase (3 mg) –No laccase | Loosen fibers, prep tissue | Only for pre-conditioning. Not recommended for resin harvest yet. |
| 4–5 years | Early resin-forming stage | –Cellulase (10 mg) – Xylanase (5 mg) –Low laccase (1–2 U/mL) | Stimulate colonization & trigger defense | Ideal time to begin inoculation protocols. Combine with Fusarium oxysporum or abiotic stress. |
| 6–7 years | Peak vascular activity | –Cellulase (10 mg) –Xylanase (10 mg) –Laccase (5 U/mL) | Maximize tissue breakdown + resin polymerization | Most responsive age for dual-action inoculants. Combine with BioFusion™. |
| 8+ years | Mature, dense wood | –High cellulase (15 mg) –High xylanase (15 mg) –Laccase (10 U/mL) –(Optional) Pectinase (5 mg) | Penetrate tough wood, accelerate dark resin buildup | May require deeper drilling. Strong response to oxidative enzyme blends. |
| Over-mature / stressed trees | Reduced vitality | –Low cellulase (5 mg) –Laccase (5 U/mL) –Peroxidase (2 U/mL) | Activate residual phenolic defenses | Use caution—monitor for overreaction or decay. Ideal for salvage resin induction. |
Standard Enzyme Dilution Guide
| Enzyme | Typical Stock Concentration | Dilution for Field Use |
|---|---|---|
| Cellulase | 1000 U/g (powder) | 1% w/v (10 mg/mL) |
| Xylanase | 1000 U/g (powder) | 0.5–1% w/v |
| Laccase | 5000 U/g or 100 U/mL | Use at 1–10 U/mL |
| Peroxidase | 100 U/mL | Use at 1–2 U/mL |
| Pectinase | 800 U/g | Use at 0.5–1% w/v |
Application Tips
- Pre-treatment (12–24 hrs before fungal/abiotic inoculant) increases enzyme effectiveness.
- Maintain pH 4.5–6.0 for optimal enzyme activity.
- Use fresh solutions; store cool and away from light.
- Enzyme blends can be delivered via injection or hydrogel carriers.