Two types of Fusarium oxysporum inoculant carriers commonly used in agarwood resin induction: one liquid-based for injection and one solid-based for wound inoculation (plug-type method).
Liquid Inoculant for Trunk Injection
Purpose: Inject directly into the xylem via syringe or infusion to induce resin production internally.
Ingredients:
| Component | Amount (per liter) | Notes |
|---|---|---|
| Distilled or clean water | 1 liter | Avoid chlorinated or contaminated water |
| Molasses (unsulfured) | 20–30 mL | Provides sugar for fungal viability |
| Glycerol (optional) | 10–20 mL | Enhances shelf life and absorption |
| Tween 20 (surfactant) | 0.5 mL (0.05%) | Improves dispersion in tree tissues |
| Fusarium oxysporum spore suspension | 10⁶–10⁸ spores/mL (approx.) | Add from lab-cultured broth |
Instructions:
- Sterilize all containers and utensils with 70% alcohol or heat.
- Mix molasses and glycerol in clean water, stir until fully dissolved.
- Add Tween 20 (optional but helps with absorption).
- Introduce the F. oxysporum spore solution under sterile conditions.
- Keep the solution at room temperature for same-day use, or refrigerate (4–10°C) for storage up to 7–10 days.
✅ Application:
- Inject 3–5 mL per tree using a syringe or pressure injector.
- Drill holes 2–3 cm deep at a downward angle.
- Seal with parafilm, beeswax, or tape if needed.
Storage & Shelf Life
- Liquid inoculants: 7–10 days in refrigeration
- Avoid sunlight or high heat to maintain fungal viability