Standard Operating Procedure (SOP) for formulating and applying Salicylic Acid (SA) Inoculantas an agarwood resin inducer in Aquilaria spp.
SOP: Salicylic Acid–Based Agarwood Inoculant
Product Name:BarIno™ SignalResin™ Tagline:“Signal the Tree. Trigger the Resin.” Purpose: To simulate a biotic stress signal and trigger systemic acquired resistance (SAR) and phenolic resin biosynthesis in agarwood trees.
1. Materials Required
Material
Specification
Salicylic Acid (SA)
Analytical or food-grade (≥99% purity)
Ethanol or Isopropanol
95% (for dissolving SA)
Distilled Water
pH 6.5–7.0
Brown Sugar (optional)
2% w/v to enhance microbial growth if co-inoculated
pH meter or pH strips
For checking final solution acidity
Beaker & Stirring Rod
Non-reactive glass or plastic
Digital Scale
For accurate weighing
PPE
Gloves, goggles, mask
2. Inoculant Formulation
Target Concentration: 0.2% w/v SA Solution
Ingredient
Quantity (per 1 Liter)
Salicylic Acid
2 grams
Ethanol (95%)
20–30 mL
Distilled Water
Up to 1000 mL
(Optional) Brown Sugar
20 g (for 2% w/v)
Preparation Steps:
Dissolve SA in Alcohol:
In a small beaker, dissolve 2 g of salicylic acid in 20–30 mL of ethanol (SA is poorly water-soluble without alcohol).
Stir until fully dissolved.
Add Water:
Pour the solution into a clean container and dilute with distilled water to make up to 1 liter.
Mix thoroughly.
(Optional) Add Sugar:
Add 20 g of brown sugar to the solution if using in combination with microbial inoculants.
Check pH:
Final pH should be between 5.5–6.5. Adjust if needed using dilute NaOH or citric acid.
3. Packaging and Storage
Parameter
Details
Container
Amber glass or HDPE bottle
Label Info
Product name, batch no., % SA, date
Shelf Life
3–5 days at room temp (10–14 days refrigerated)
Storage Conditions
Cool, dark, and dry place
4. Field Application Guidelines
Parameter
Specification
Tree Age
≥ 4 years (Aquilaria malaccensis, etc.)
Injection Hole Size
6–8 mm diameter, 5–7 cm deep
Hole Spacing
10–15 cm vertical intervals
Volume per Hole
3–5 mL per hole
Application Method
Gravity drip, pressure injector, or syringe
Repeat Frequency
Every 6–12 months if needed
5. Safety Precautions
Use gloves and goggles when handling salicylic acid and ethanol.
Avoid inhalation or skin contact.
Keep away from heat or flame (ethanol is flammable).
Dispose of unused or expired solution per local regulations.
