Microbial Enzyme | BioFusion™ | BioFusion™ Formulation | BioFusion™ Development | BioFusion™ Profile
Development timeline for BarIno™ BioFusion™, a microbial enzyme-based inoculant designed to promote premium agarwood resin formation through a biological pathway. This inoculant works by colonizing Aquilaria malaccensis tissues with select microbes that secrete natural enzymes and signal molecules to stimulate endogenous defense-related resin production.
BarIno™ BioFusion™ Monthly Development Timeline
(For Premium Agarwood Resin Induction)
Application Age Target: Aquilaria malaccensis ≥5 years / ≥10 cm DBH
Initial Dose: 10–20 mL per site
Reinoculation: Optional every 6–9 months
| Month | Biological Activity | Tree Observation | Action Point |
|---|---|---|---|
| Month 0 | Inoculation Phase:Microbial colonization of xylem tissue begins. Enzymes (e.g., cellulases, oxidases) introduced. | Mild wound response at inoculation site. No visual changes in heartwood. | Drill inoculation holes; inject BarIno™ BioFusion™; plug if needed. |
| Month 1 | Microbial establishment in tissue.Low-level host oxidative and salicylic acid pathways activated. | Tree remains healthy; slight swelling or browning near site. | Inspect for infection or overgrowth. No reapplication needed. |
| Month 2 | Enzymatic degradation of internal barriers begins. Tree responds by compartmentalization and forming oil-rich zones. | Some internal browning visible in bored holes; no external resin. | Optional: drill & sample for early heartwood changes. |
| Month 3 | Sustained microbial stimulation.Initial resin-like compounds start forming in parenchyma tissues. | Heartwood starts showing faint scent or coloration. | Evaluate using test core or aromatic oil strip. |
| Month 4 | Metabolic intensification:Secondary metabolites (e.g., sesquiterpenes) increase. | Inoculated zone may emit light agarwood scent. | Midpoint evaluation: optional lab GC-MS or FTIR sample. |
| Month 5–6 | Core Resin Formation Phase:Coloration intensifies (brown to black); oil pockets form. | Start seeing true agarwood streaks on split core. | Optional: reinforce with microbial nutrient booster or fungal synergist. |
| Month 7–8 | Maturation:Resin diffuses beyond inoculation sites. Tree stabilizes response. | Resin-scented core present in cross-sections. Scent strength increases. | Consider reinforcing weak responders with 2nd inoculation. |
| Month 9–12 | Harvest Potential Window:Core can be sampled for essential oil distillation or chip grading. | High-grade resin zones observable. Harvest window opens. | Tree-specific decision: early harvest or prolong for denser resin. |
Optional Enhancements
- Apply BarIno™ BioBooster™ (a prebiotic/molasses-based enhancer) at Month 2 or Month 5 for faster colonization.
- Use GC-MS or FTIR analysis at Month 4 and Month 8 to verify sesquiterpene buildup.