TriSalix™ Development

Chemical Induction | TriSalix™ | TriSalix™ Formulation | TriSalix™ Development | TriSalix™ Profile

Monthly Development Timeline for agarwood formation following inoculation with BarIno™ TriSalix™ (FeCl₃ + MgCl₂ + NaCl), designed for Aquilaria malaccensis trees under Philippine agroclimatic conditions:

BarIno™ TriSalix™ Agarwood Development Timeline

Inoculant Type: Abiotic Triple-Salt Inducer
Mode of Action: Osmotic + Oxidative Stress Trigger
Inoculation Method: Trunk injection (zigzag/spiral)

Month 0 – Inoculation

• Action: Injection of TriSalix™ (5–10 mL per hole, 20 cm spacing, 2–3 cm depth).
• Tree Condition: Minimum 5 years old, ≥10 cm DBH.
• Signs: Slight leaf yellowing or stress response (temporary).
• Note: Begin environmental stress logging (rainfall, humidity, temp).

Month 1 – Cellular Shock Response

• Physiological Response:
  • Increased electrolyte leakage at wound sites.
  • Initiation of reactive oxygen species (ROS) signaling.
  • Cambium and xylem stress metabolite production begins.
• Observable Signs:
  • Discoloration around injection holes (light brown to rusty red).
  • Minor callus formation.

Month 2 – Resin Induction Phase

• Activity:
  • Tree cells undergo apoptosis near injection zone.
  • Initial resin precursors form (phenolics, sesquiterpenes).
• Signs:
  • Inner wood staining begins to deepen to amber/light brown.
  • Stronger, musky aroma upon drilling into affected area.

Month 3–4 – Active Resin Biosynthesis

• Metabolic Shift:
  • Fe³⁺ + Mg²⁺ ions stimulate enzyme pathways (e.g., terpene synthases).
  • NaCl-induced osmotic imbalance enhances resin saturation.
• Result:
  • Sticky or tar-like resin appears in central core (oil-rich zone).
• Wood Color:
  • Transition to dark brown or olive-colored streaks around borehole.

Month 5–6 – Resin Expansion

• Spread:
  • Resin zone expands laterally (1–2 cm from injection tunnel).
  • Induction site enlarges with vertical spread in conductive tissues.
• Monitoring Tip:
  • Use increment borer or drilled sample to observe ring progression.

Month 7–8 – Partial Maturation

• Maturation Stage:
  • Resin polymerization begins — more complex aromatic compounds form.
  • Lignification of wounded tissue strengthens.
• Wood Hardness:
  • Noticeable increase in density at infected zone.

Month 9–10 – Resin Hardening & Scent Intensification

• Final Color:
  • Blackish-brown core visible in longitudinal cuts.
  • Aroma more pronounced (incense, medicinal, balsamic).
• Harvest Readiness Checkpoint 1:
  • Perform chip or core test for resin density and yield ratio.

Month 11–12 – Harvest-Ready Evaluation

• Options:
  • For partial harvest: Remove infected limb or segment.
  • For full harvest: Fell tree if >50% of trunk shows resin zone.
• Indicators:
  • ≥15–20% dark core yield in cross-section.
  • Strong oud scent without fungal overgrowth.

Post-12 Months: Re-Inoculation/Extension

• Re-inoculation on alternate side of trunk can be done after 12–15 months.
• Combine with microbial booster (e.g., BarIno™ BioFusion™) for dual-mode inoculation.

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