Chemical Induction | TriSalix™ | TriSalix™ Formulation | TriSalix™ Development | TriSalix™ Profile
Standard Operating Procedure (SOP)
Product Name: BarIno™ TriSalix™
Type: Triple-Salt Chemical Inoculant for Agarwood Induction
Active Ingredients: Ferric Chloride (FeCl₃), Magnesium Chloride (MgCl₂), Sodium Chloride (NaCl)
Form: Water-based injectable solution
Batch Volume: 1 Liter
I. PURPOSE
To prepare a stable, effective aqueous formulation of BarIno™ TriSalix™, a non-biotic, abiotic stress inoculant that mimics natural resin-inducing environmental stresses (oxidative, osmotic, ionic) to trigger agarwood formation in Aquilaria trees.
II. MATERIALS & CHEMICALS
| Component | Chemical Formula | Purity | Function | Amount (per 1L) |
|---|---|---|---|---|
| Ferric Chloride | FeCl₃·6H₂O | Analytical | Oxidative stress agent | 7.5 g (0.75% w/v) |
| Magnesium Chloride | MgCl₂·6H₂O | Analytical | Enzymatic cofactor & osmotic stressor | 7.5 g (0.75% w/v) |
| Sodium Chloride | NaCl | Food/lab grade | Osmotic stress agent | 15.0 g (1.5% w/v) |
| Distilled Water | H₂O | – | Solvent & carrier | To make 1000 mL |
⚠️ Use only clean, non-reactive (glass or HDPE) equipment.
III. FORMULATION PROCEDURE
- Prepare Work Area:
- Sanitize all containers, tools, and workspace.
- Wear PPE (gloves, goggles, apron).
- Initial Dissolution:
- In a 1-liter capacity glass beaker or flask, pour 700 mL of distilled water.
- Begin stirring gently using a magnetic stirrer or sterile glass rod.
- Add Sodium Chloride (NaCl):
- Add 15.0 grams slowly.
- Stir until fully dissolved (2–3 minutes).
- Add Magnesium Chloride (MgCl₂·6H₂O):
- Add 7.5 grams gradually.
- Stir thoroughly until the solution clears (3–5 minutes).
- Add Ferric Chloride (FeCl₃·6H₂O):
- Slowly add 7.5 grams.
- This may cause a slight yellowish or light brown hue.
- Stir for 5–10 minutes until fully dissolved.
- Top-Up with Water:
- Add distilled water to reach exactly 1000 mL.
- Mix thoroughly for an additional 5 minutes.
- Check pH (optional):
- Ideal pH range: 4.5–5.5
- Adjust slightly with dilute NaOH or HCl if needed.
- Bottle Immediately:
- Transfer into sterilized amber glass or HDPE bottles.
- Seal tightly to prevent oxidation.
IV. STORAGE & STABILITY
- Storage Temp: 15–30°C, away from sunlight
- Shelf Life: 6 months (unopened), 30 days (opened)
- Container: Amber bottle or opaque HDPE
- Signs of instability: Precipitation, foul odor, or discoloration
V. FIELD APPLICATION INSTRUCTIONS
| Parameter | Description |
|---|---|
| Target Trees | Aquilaria malaccensis ≥ 5 years old |
| Injection Volume | 5–10 mL per hole |
| Hole Depth | 2–3 cm into xylem |
| Hole Pattern | Spiral or zigzag up the trunk (15–20 cm apart) |
| Tool | Cleaned hand drill or inoculation gun |
| Repeat Cycle | Optional re-application after 6–12 months |
VI. SAFETY PRECAUTIONS
- Handle with gloves and protective eyewear.
- Avoid skin and eye contact.
- Do not ingest or inhale vapors.
- Dispose of excess chemicals and containers according to DENR–EMB guidelines.