Microbial Enzyme | FusaBlaze™ | FusaBlaze™ Formulation | FusaBlaze™ Development | FusaBlaze™ Profile
Development timeline for BarIno™ FusaBlaze™, a Fusarium oxysporum–based microbial enzyme inoculant designed to induce high-quality agarwood resin formation. This timeline outlines the expected stages of resin biosynthesis and tree response following inoculation.
BarIno™ FusaBlaze™ Monthly Development Timeline
Fusarium oxysporum-Based Microbial Enzyme Induction for Premium Agarwood
Month 0: Inoculation Phase
- Drilling & Injection
- 6–8 mm holes drilled in spiral or ring pattern (15–20 cm spacing).
- 10–20 mL FusaBlaze™ injected per hole.
- Initial Colonization Begins
- F. oxysporum spores begin to colonize internal tissues around the inoculation point.
Month 1–2: Host-Microbe Interaction Phase
- Endophytic Activity
- Fungus grows within xylem and parenchyma tissue, releasing elicitor proteins and ligninolytic enzymes(peroxidases, cellulases, etc.).
- Mild Localized Stress
- Plant defense pathways activate due to perceived microbial threat.
- Early production of precursor compounds (phenolics, terpenoids).
Month 3–4: Initial Resin Induction Phase
- Secondary Metabolite Activation
- Tree produces antimicrobial resins as a defense mechanism.
- Begins converting primary metabolites into sesquiterpenes and chromones (key resin compounds).
- Internal Color Changes
- Brown to darkening streaks appear near inoculation sites.
Month 5–6: Resin Expansion Phase
- Wounding Response Spreads
- Resin formation spreads longitudinally and radially around the inoculation site.
- Wood in affected areas begins to harden and develop aromatic properties.
- Volatile Oil Production Begins
- Oils and aroma compounds begin forming in small quantities.
Month 7–9: Resin Maturation Phase
- Visible Signs of Resinization
- Affected wood shows darker, denser areas with visible resin concentration.
- Aroma Development
- Distinct sweet, woody, balsamic scent may be noticed near sites.
Month 10–12: Peak Formation Phase
- High-Quality Agarwood Resin Formed
- Accumulation of oleoresins, chromone derivatives, and sesquiterpenes reaches optimal level.
- Harvest Window Opens
- Trees may be evaluated (via drilling cores or scent checks) for readiness for partial or full harvest.
Post-12 Months: Optional Reinoculation / Monitoring
- Reinoculation Possible
- Second round may be applied to promote further resin formation or new zones of induction.
- Monitoring & Resin Quality Testing
- Assess via ethanol extraction, distillation, or spectrometry for chromone/sesquiterpene content.
Summary Chart
| Month | Phase | Key Activities & Observations |
|---|---|---|
| 0 | Inoculation | Application of FusaBlaze™, fungal colonization begins |
| 1–2 | Colonization | Enzyme release, mild stress, host recognition |
| 3–4 | Induction | Start of resin biosynthesis, internal color change |
| 5–6 | Expansion | Widening resin zone, volatile oil formation begins |
| 7–9 | Maturation | Aroma and visible resin deepen |
| 10–12 | Peak Formation | Harvest readiness, chromone buildup |
| 12+ | Optional Follow-Up | Reinoculation or tree rest period |