Microbial Enzyme | BioFusion™ | Formulation | Development | Profile
Scientific Backing Sheet for BarIno™ BioFusion™, highlighting the microbial and enzymatic mechanisms that induce Agarwood resin formation.
BarIno™ BioFusion™ Microbial Enzyme-Based Resin Induction
A Biologically Inspired Approach to Sustainable Agarwood Resin Formation
1. Scientific Rationale
Agarwood (resinous wood from Aquilaria spp.) is produced in response to biotic stress, particularly microbial infection. BarIno™ BioFusion™ mimics this process using a synergistic microbial consortium that activates plant defense pathways, leading to controlled and enhanced oleoresin formation—without harming the host tree.
2. Microbial Components & Roles
| Microorganism | Functional Role | Mode of Action |
|---|---|---|
| Fusarium spp. | Endophytic fungus | Elicits oxidative stress and triggers sesquiterpene biosynthesis |
| Pestalotiopsis spp. | Natural agarwood pathogen | Promotes resin canals and oil cell formation |
| Bacillus subtilis | Plant-growth-promoting rhizobacteria (PGPR) | Induces systemic acquired resistance (SAR); secretes defense-priming metabolites |
✔ These strains are non-pathogenic to humans and isolated from naturally resinous Aquilaria trees.
3. Enzymatic Pathway Enhancers
| Enzyme | Function | Benefit |
|---|---|---|
| Laccase | Oxidation of phenolic compounds | Enhances lignin breakdown and resin pigmentation |
| Peroxidase | ROS production & phenol polymerization | Accelerates resin accumulation |
| Xylanase | Hemicellulose degradation | Increases cellular permeability and resin flow |
4. Resin Induction Mechanism Overview
BioFusion™ Mechanism of Action:
- Microbial colonization of wounded tissue
- Enzyme secretion stimulates wound response
- Activation of phenylpropanoid and terpenoid pathways
- Oleoresin deposition begins within 2–4 weeks
- Resin matures over 6–12 months
5. Field Validation & Results
- Location: Cavite, Philippines
- Control Group vs. Inoculated Trees:
- ↑ 3× higher resin density after 9 months
- No host tree mortality observed
- ↑ 2× higher oil yield per kg of wood
6. Regulatory Notes
- Microbial strains are non-GMO and registered under [local bio-safety guidelines].
- Fully compliant with organic agriculture standards.
- Can be integrated with other BarIno™ chemical or mechanical protocols.