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Standard Operating Procedure (SOP) for formulating and applying a Hydrogen Peroxide (H₂O₂) Inoculant for agarwood induction in Aquilaria spp.
SOP: Hydrogen Peroxide – Based Agarwood Inoculant
Product Name: BarIno™ OxiWound™
Tagline: “Burn. Bleed. Bloom.”
Purpose: Induce oxidative stress to promote agarwood resin formation.
1. Materials Required
| Item | Specification |
|---|---|
| Hydrogen Peroxide (H₂O₂) | Food/medical-grade (3%–10% solution) |
| Distilled Water | To dilute concentrate (if needed) |
| Brown Sugar (optional) | 1–2% w/v (microbial support if co-inoculated) |
| Measuring Cylinder | For liquid volume measurement |
| Plastic Beaker | For mixing |
| Protective Gear | Gloves, safety glasses, mask |
| Storage Bottle | Amber HDPE bottle with label |
2. Inoculant Formulation
Target Working Concentration: 5% w/v H₂O₂ Solution
If using 10% stock H₂O₂:
- Mix 500 mL of 10% H₂O₂ with 500 mL distilled water
If using 30% stock H₂O₂ (laboratory grade):
- Mix 167 mL of 30% H₂O₂ with 833 mL distilled water
(Optional: Add 20 g brown sugar per liter to promote microbial colonization)
Do not mix hydrogen peroxide with acidic solutions (like vinegar or citric acid) as this may release toxic vapors or degrade the H₂O₂.
3. Packaging and Storage
| Parameter | Details |
|---|---|
| Container | Dark HDPE or amber glass bottle |
| Label Info | Product name, concentration, date, warnings |
| Storage Life | Up to 10 days refrigerated; 3–5 days room temp |
| Keep Away From | Heat, light, and organic materials |
4. Field Application Protocol
| Step | Description |
|---|---|
| 1 | Select healthy, ≥4-year-old Aquilaria trees |
| 2 | Drill boreholes: 6–8 mm diameter, 5–7 cm deep |
| 3 | Space holes vertically every 10–15 cm |
| 4 | Inject 3–5 mL of 5% H₂O₂ per hole |
| 5 | Watch for bubbling (oxidation reaction = active tissue) |
| 6 | Optionally insert soaked cotton or wick for slow release |
| 7 | Seal holes if desired (optional, with parafilm or wax) |
5. Safety Precautions
- Wear gloves, goggles, and a mask while handling H₂O₂
- Avoid contact with skin, eyes, and clothing
- Store away from heat, light, and metal containers
- Do not mix with acids, flammables, or unknown chemicals
- Use only in well-ventilated areas or outdoors
6. Example Log Sheet Entry
| Date | Tree ID | Dose | H₂O₂ % | Notes (foaming, color change, aroma) |
|---|---|---|---|---|
| 2025-06-08 | AQ-001 | 5 mL | 5% | Moderate foaming, browning after 3 days |